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Pathogenic DNA/ RNA Detection Kits


CodePathogens Qty (rxns)Ref
QRT-001 (a/b/c)Avian Flu (H5N1)25/50/1001
PCRF-001Borrelia burgdorferi80/160/3204
QRT-101 (a/b/c)Bibrio parahemolyticus48/96/1922
QRT-002 (a/b/c)Burkholderia pseudomalie25/50/1001
QRT-102 (a/b/c)Campylobacter48/96/1922
PCRF-002Campylobacter coli, lari, jejuni80/160/3204
QRT-003 (a/b/c)Chikungunya25/50/1001
QRT-004 (a/b/c)Chlamydia pneumonia25/50/1001
STR-001 (a/b/c)CAP Bacterial12/60/1203
STR-002 (a/b/c)CAP Viral12/60/1203
STR-003 (a/b/c)CAP Resistance12/60/1203
STR-004 (a/b/c)CAP Juvenile12/60/1203
PCRF-003Clostridium difficile80/160/3204
QRT-005 (a/b/c)CMV25/50/1001
PCRF-004Cryptosporidium parvum80/160/3204
QRT-006 (a/b/c)Dengue25/50/1001
PCRF-005Entamoeba histolytica80/160/3204
QRT-103 (a/b/c)Enterobactor sakazaklii48/96/1922
QRT-007 (a/b/c)Enterovirus 25/50/1001
QRT-104 (a/b/c)E.Coli O157:H748/96/1922
QRT-008 (a/b/c)Filaria25/50/1001
PCRF-006Giardia lamblia
QRT-009 (a/b/c)Hanta25/50/1001
QRT-010 (a/b/c)Helicobactor pylori25/50/1001
PCRF-007Helicobactor pylori80/160/3204
QRT-011 (a/b/c)HAV 25/50/1001
QRT-012 (a/b/c)HBV 25/50/1001
QRT-013 (a/b/c)HCV 25/50/1001
QRT-014 (a/b/c)HEV 25/50/1001
QRT-200 (a/b/c)HEV25/50/1004
QRT-015 (a/b/c)HIV-1 25/50/1001
STR-005 (a/b/c)HPV Typing 12/60/1203
STR-006 (a/b/c)HPV Screening 12/60/1203
QRT-016 (a/b/c)HSV 1+225/50/1001
STR-007 (a/b/c)HSV 1+212/60/1203
QRT-017 (a/b/c)H.Influenza25/50/1001
STR-008 (a/b/c)Influenza Typing12/60/1203
STR-009 (a/b/c)Influenza Screening12/60/1203
STR-010 (a/b/c)Influenza H5N112/60/1203
QRT-018 (a/b/c)JEV  25/50/1001
QRT-201 (a/b/c)JC/BK25/50/1001
PCRF-008Legionella pneumophila80/160/3204
QRT-019 (a/b/c)Leprosy25/50/1001
QRT-020 (a/b/c)Leptospira (pathogenic)25/50/1001
QRT-104 (a/b/c)Listeria monocytogene48/96/1922
PCRF-009Listeria monocytogene80/160/3204
QRT-021 (a/b/c)Malaria25/50/1001
QRT-022 (a/b/c)Measles Virus25/50/1001
PCRF-010Microsporidium80/160/3204
PCRF-011Mycoplasma tuberculosis80/160/3204
PCRF-012Mycoplasma pneumophila80/160/3204
STR-011 (a/b/c)MRSA Control12/60/1203
QRT-023 (a/b/c)MTb complex25/50/1001
QRT-024 (a/b/c)MTb Complex/MOTT25/50/1001
QRT-025 (a/b/c)N.Meningitis25/50/1001
PCRF-013Norovirus80/160/3204
STR-012 (a/b/c)Periodontitis 12/60/1203
QRT-202 (a/b/c)Rabies25/50/1001
QRT-105 (a/b/c)Salmonella48/96/1022
PCRF-014Salmonella80/160/3204
QRT-026 (a/b/c)SARS25/50/1001
QRT-105 (a/b/c)Shigella48/96/1922
PCRF-015Shigella flexneri80/160/3204
QRT-027 (a/b/c)Scrub Typhus25/50/1001
QRT-106 (a/b/c)Staphylococcus aureaus48/96/1922
STR-013 (a/b/c)Sexual Transmitted Diseases (STD)12/60/1203
QRT-028 (a/b/c)Streptococcous pneumonia25/50/1001
QRT-029 (a/b/c)TTV25/50/1001
QRT-107 (a/b/c)V.cholera48/96/1922
QRT-108 (a/b/c)V.cholera O148/96/1922
QRT-109 (a/b/c)V.cholera O13948/96/1922
PCRF-016VTEC st1/stx280/160/3204
QRT-030 (a/b/c)West Nile Virus25/50/1001
PCRF-017Yersinia enterocolitica80/160/3204

All kits (1) are quantitative real time and optimized on Rotor 2000/3000/6000 Gene Machines

All kits (2) are quantitative real time and optimized on Light Cycler Machine

All kits (3) are PCR/hybridization assays on the strips:

STR-001 (a/b/c)   Detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila

STR-002 (a/b/c)   Detection of 7 most common viruses causing pneumonia: Influenza virus A & B, Parainfluenza virus (PIV) 1, PIV 2, PIV 3, Respiratory Syncytial virus (RSV) and Adenovirus 

STR-003 (a/b/c)   Detection of Streptococcus pneumoniae and its antibiotic resistances 

STR-004 (a/b/c)   Detection of Streptococcus pneumoniae, Haemophilus influenzae, Bordetella pertussis and Bordetella parapertussis 

STR-005 (a/b/c)   Detection of HPV and differentiation in "high risk" and "low risk" genotypes. Subtyping of the most important high risk virus types HPV 16 , HPV 18 as well as subtyping of HPV 6 and 11 and of high risk types of the 30, 40 and 50 group

STR-006 (a/b/c)   Detection of HPV and differentiation in "high risk" and "low risk" genotypes

STR-007 (a/b/c)   Detection of both Herpes simplex subtypes including an human amplification control

STR-008 (a/b/c)   Detection of Influenza A Virus and differentiation in Influenza A Virus hemagglutinin subtypes H1, H3, H5, H7 and H9 

STR-009 (a/b/c)   Differentiation in Influenzavirus A and B as well as Parainfluenza 1, 2 and 3 

STR-010 (a/b/c)   Differentiation in Influenza A and B infections and detection of H5 and N1 

STR-011 (a/b/c)   Detection of Staphylococcus aureus and its Macrolide, Beta-Lactame, Tetracycline, Aminoglycoside and Quinolone resistance genes

STR-012 (a/b/c)   Detection of Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Prevotella intermedia , Bacteroides forsythus und Treponema denticola as well as the predisposing HLA-DR4 alleles 

STR-013 (a/b/c)   Detection of the most common causes of genital infections: HPV , Herpes simplex Virus Type 1 and 2 , Chlamydia trachomatis , Neisseria gonorrhoea and Treponema pallidum


All kits (4): Standard kits for Human Stool, Sputum/Throat Swab, Synovial fluid analysis with systems of Gel/Real-time SYBER Green. Related DNA extraction Kits are available.



Other formats of detection such by agraose gel  (5) or other machines, consumables for molecular biology are also available on request. 

Asking for Technical Information and Package Insert







Example of  Selected Kit Information from Package Insert (1):

Product Name:  HEV Real Time PCR Kit - Opimized for Rotor Gene Machine* (Cat.No.QRT-014C)


1. Contents of the Kit:

Color CodeContents25 rxns
R1 BlueHEV Super mix25 rxns x 1 Vials
R2 YellowMg Sol RT. 1 Vial
HEV-S1 Red HEV Standard 1 ; 1 X 105 copies/µl 1 Vial of 300µl
HEV-S2  Red
HEV Standard 2 ; 1 X 104 copies/µl
1 Vial of 300µl
HEV-S3 Red
HEV Standard 3 ; 1 X 103 copies/µl 1 Vial of 300µl
HEV-S4 Red HEV Standard 4 ; 1 X 102 copies/µl 1 Vial of 300µl
HEV-S5 Red HEV Standard 5 ; 1 X 101 copies/µl 1 Vial of 300µl
W White Molecular Grade Water. 1 Vials of 1 ml
IC-1 (R3) GreenIC-1 RG (R3) 1 Vial of 1 ml

R = Reagents
S = Quantitation Standards
W = Molecular Grade Water.

All Vials have Color Coder tops to distinguish between different reagents.

2. Storage of the Kit.

All the reagents of the kit should be stored at -20°C and is stable till expiry at this
temperature. Repeated thawing and freezing (> 3x) should be avoided, as this may
reduce the sensitivity of the assay. If the kit is to be used only occasionally, the
reagents should be frozen in aliquots. Storage at +4°C is not recommended & should
not exceed a period of 2 hours in any case.

* The Rotor Gene™ 2000/3000 is a registered trademark of Corbett Research, Australia.

3. HEV Information - Application

Hepatitis E is a liver disease caused by the hepatitis E virus (HEV) transmitted
in much the same way as hepatitis A virus. Hepatitis E virus (HEV) infection
results in hepatitis E, an acute and self-limited disease. The virus is
transmitted in a faecal–oral manner and is a major cause of viral hepatitis in
much of the developing world, where it causes rampant sporadic infections
and large epidemics. A curious feature of hepatitis E is the unusually high
rates of mortality that are observed in pregnant women, in whom the disease is
exacerbated by the development of fulminant liver disease. In the absence of
viable in vitro propagation systems, several geographical isolates of HEV have
been maintained in vivo in nonhuman primates and, subsequently, the viral
genome has been cloned and sequenced. HEV has been classified
provisionally into a separate family known as the HEV-like viruses, which has
at least four recognised genotypes, but has only a single serotype. The viral
genome is a positive-stranded (+) RNA of ~7.5 kb and encodes at least three
proteins. Open reading frame 1 (ORF1) encodes the viral nonstructural
polyprotein, which has domains that are homologous to some of the
replication and processing enzymes found in other +RNA viruses. The HEV
protein itself remains poorly Characterised. The protein encoded by open
reading frame 2 (ORF2) is the major HEV capsid protein, and the protein
encoded by open reading frame 3 (ORF3) appears to be involved in virus–host
interactions. Several questions related to the biology, epidemiology and
pathogenesis of HEV remain unanswered as yet.

The HEV Quantification assay is developed for laboratory scale or high-
throughput quantitative transcript analysis by real time quantitative fluorescence
PCR.

The standardized ready-to-use Control and Reaction mix allow fast
processing of the samples.

Samples which can be used for Extraction: Serum, plasma, whole blood, stool
etc.


4. Precautions for PCR

The following aspects should always be taken care of:

• Store positive material (Specimens, Standards or amplicons) separately from
all other reagents and add it to the reaction mix in a separate facility.
• Thaw all components thoroughly at room temperature before starting the
assay.
• When thawed, mix the components and centrifuge briefly.
• Work quickly on ice or in the Cooling Block.

• All the reagents including the NTC (except for standards & specimens) should
be mixed & dispensed in pre-mix area.
• All the standards & specimens should be mixed & dispensed in extraction
area.
• Use pipette tips with filters only.
• Always use disposable powder-free gloves

5. Additionally Required Materials and Devices

• RNA isolation kit (see 8.a. RNA extraction)
• 0.2 ml PCR tubes for use with 36-well rotor (Corbett Research, Cat.-Nr.: SE-
1003F) alternatively 0.1 ml PCR tubes for use with 72-well rotor (Corbett
Research, Cat.-Nr.: ST-1001)
• Micro Pipettes Variable Volume 2-20µl, 10-100µl, 100-1000µl,
• Sterile pipette tips with aerosol barrier 2-20µl, 10-100µl, 100-1000µl,
• Disposable powder-free gloves
• Vortex mixer
• Centrifuge Desktop with rotor for 1.7 ml reaction tubes
• Rotor Gene™ 2000 or Rotor Gene™ 3000, Corbett Research (The Real time
PCR Instrument)

6. Principle of Real-Time PCR

The robust assay exploits the so-called Taqman principle. During PCR, forward and
reverse primers hybridize to a specific sequence product. A TaqMan probe, which is
contained in the same reaction mixture and which consists of an oligonucleotide
labeled with a 5'-reporter dye and a downstream, 3'-quencher dye, hybridizes to a
target sequence within the PCR product. A Taq polymerase which possesses 5' - 3'
exonuclease activity cleaves the probe. The reporter dye and quencher dye are
separated upon cleavage, resulting in an increase in fluorescence for the reporter.
Thus, the increase in fluorescence is directly proportional to the target amplification
during PCR.

7. Description Of the Product.

The HEV PCR Reagents constitute a ready to use system for detection
and quantification of HEV using Polymerase chain reaction (PCR) in the Rotor Gene
2000/3000 (Corbett Research). The Specific Master mix contains reagents and
enzymes for the specific amplification of HEV and for the direct detection of the
specific amplicon in fluorescence channel Cycling A. FAM of the Rotor Gene
2000/3000 & the Reference gene on Cycling A. Joe. External positive Standards
(HEV S 1-5) are supplied which allow the determination of the gene load. For further
information, please refer to section 8.c Quantitation of the package insert.


Figure: Detection of the quantitation standards (HEV S 1-5) in fluorescence channel
Cycling A.FAM. NTC: non-template control.  Examples of positive and negative PCR reactions are given in the above figure.



PCR Related Materials, other PCR Kits, Viruses