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Influenza
AB-AI-001
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ELISA-IF-Ab-AB
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Flu A Ab ELISA
Flu A Ag ELISA
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H5: Ab ELISA
H5: Ag ELISA
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H5: Antibody
H5: Antigen
H5: Gene Test
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Anfahrt




Product Name: H5N1 Gene Test

Product Code:  HN-0001

Intended Use

The kit is produced for the determination of viral RNA from influenza A/H5N1 using reverse transcriptase/polymerase chain reaction.

Assay Principle

Influenza virus infection is a major public health problem worldwide. Influenza viruses are enveloped, single stranded RNA viruses of the Orthomyxoviridae family. They are classifed as types A, B, and C based on differences in their nucleoproteins and matrix proteins. The influenza A/H5N1 strain is found as highly pathogenic (HPAI) as well as low pathogenic (LPAI) viruses.

The kit is designed for the detection of viral RNA from influenza A/H5N1. The kit contains the lyophilised reaction mixes Rx-Mix A, Rx-Mix H5 and Rx-Mix N1. Rx-Mix A allows the determination of the type of influenza virus, i.e. A. Rx-Mix identified the hemagglutinin gene HA 5. The reaction mix N1 characterises the neuraminadase gene NA1.

Target RNA and Amplified Fragments

Viral RNA from Influenza A virus

  • Amplicon size with A-Mix: 191 bp
  • Amplicon size with H5-Mix: 192 bp
  • Amplicon size with N1-Mix: 107 bp


Kit Contents

  • Reaction mix (Rx-Mix A), lyoph. for 5 x 10 assays
  • Reaction mix (Rx-Mix H5), lyoph. for 5 x 10 assays
  • Reaction mix (Rx-Mix N1), lyoph. for 5 x 10 assays
  • Sterile water  (PCR grade)              15 x 500µl

The kit contains the lyophilised reaction mixes for running 50 specific amplifications respectively, after reconstitution. Rx-Mix A allows the determination of the type of influenza virus, i.e. A. Rx-Mix H5 identifies the hemagglutinin gene HA 5. The reaction mix N1 characterises the neuraminadase gene NA1.

Required Materials not provided

  • Taq-Polymerase (0.5U/assay required)
  • Reverse Transcriptase (5U/assay required)
  • Agarose for preparing a 3% gel (can be included if required)
  • TAE- or TBE electrophoresis buffer (can be included if required)
  • Sample Buffer (can be included if required)
  • DNA-Standard (can be included if required)
  • Ethidiumbromide or other dyes for nucleic acid staining
  • Mineral oil for thermocyclers without heating lid.