Antigens for immunological assays (EIA, Western Blot, IHA, Line-assays, etc) are prepared from recombinant proteins, infected cell cultures or microorganisms grown in axenic media (bacteria, parasites, etc). they are partially purified and are supplied lyophilized or frozen. They may contain NaN3 or thimerosal. Refer to the Certificate of analysis Sheet for details.
For cells lines and strains used, please request separate list.
Code
Product Description
Source
Format
Qty
MYAG-001
Mycoplasma gallisepticum Antigen
recombinant
purified
1mg
MYAG-002
Mycoplasma hominis Surface Antigen p120
recombinant
purified
1mg
MYAG-003
Mycoplasma pneumoniae Antigen
Broth base medium
purified
1ml
Mycoplasma pneumoniae Antigen
Product Code
MYAG-003
Agent
Mycoplasma pneumoniae. Strain FH. NCTC 10119
Culture System
Broth culture
Presentation
Harvested organisms are washed and processed in a two step detergent extraction process. Antigen consists of a lysate of organisms enriched for P1 adhesion protein in phosphate solubilising buffer containing 2% n-octylglucopyranoside
Inactivation
By detergent treatment. Confirmation by attempted growth under original culture conditions
Preservation
None
Protein Concentration
mg/ml by Bradford (Biorad) assay using BSA as standard
Storage
-20 to -80°C. Repeated freezing and thawing should be avoided