TORCH agents cause a varied spectrum of disease. Advances in ultrasound, invasive perinatal procedures and molecular diagnostics have allowed in utero evaluation. Infected fetuses, especially those which are sonographically abnormal, may be treated in utero depending upon the pathogen and attendant pathophysiology. Subclinical perinatal infections may lead to later childhood deficits. Such infected fetuses may benefit from early diagnosis and prompt initiation of rehabilitative measures.

Antigen upto gram scale of above-mentioned are available. Please require. Thanks.

Cytomegalovirus Antigen

Product Code                      
CMV-AG01

Agent            
Cytomegalovirus. Strain Towne. ATCC VR-977

Culture System                  
Human Embryo Lung cell culture

Presentation           
Infected cells are harvested, washed and virus extracted by alkaline treatment and sonnication. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in 0.1M glycine buffer, pH 9.6

Inactivation             
Beta-propiolactone treatment. Confirmation by attempted growth under original culture conditions

Preservation           
None 

Protein Concentration                   ´
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Herpes Simplex Virus Type 1

Product Code                     
HSV-AG02

Agent            
Herpes Simplex Virus Type 1. Strain F. ATCC VR-733

Culture System                  
Vero cell culture

Presentation           
Virus extracted from infected cells is concentrated by ultracentrifugation and then partially purified by centrifugation through sucrose. The antigen is resuspended and homogenised. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in 0.1M glycine buffer pH 9.6

Inactivation             
Beta-propiolactone treatment. Confirmation by attempted growth under original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Herpes Simplex Virus Type 2

Product Code                     
HSV-AG04

Agent            
Herpes Simplex Virus Type 2. Strain GF. ATCC VR-734

Culture System                  
Vero cell culture

Presentation           
Virus extracted from infected cells is concentrated by ultracentrifugation and then partially purified by centrifugation through sucrose. The antigen is resuspended and homogenised. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in 0.1M glycine buffer pH 9.6

Inactivation             
Beta-propiolactone treatment. Confirmation by attempted growth under original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Measles Virus Antigen

Product Code                      
MSL-AG01

Agent            
Measles Virus. Strain Edmonston ATCC VR-24

Culture System                  
Vero cell culture

Presentation           
Infected cells and culture supernatant are harvested and concentrated by ultracentrifugation. Following sonnication and clarification the resulting suspension is again concentrated by centrifugation. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in glycine buffer, pH 9.6.

Inactivation             
Beta-propiolactone treatment. Confirmation by attempted growth under original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Rubella Virus Antigen (Grade 2)

Product Code                     
RUB-AG01

Agent            
Rubella Virus Strain Judith

Culture System                  
Vero cell culture

Presentation           
Tween-ether treated concentrated infected cell culture medium

Inactivation             
Tween-ether treatment
Confirmation by attempted growth under
original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided 

Rubella Virus Antigen (Grade 1) IgM

Product Code                     
RUB-AG02

Agent            
Rubella Virus Strain Judith

Culture System                  
Vero cell culture

Presentation           
Concentrated infected cell culture medium purified by sucrose density gradient centrifugation followed by Tween-ether treatment

Inactivation             
Tween-ether treatment
Confirmation by attempted growth under
original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided 

Toxoplasma gondii IgG Antigen

Product Code                      
TOX-AG01

Agent            
Toxoplasma gondii Strain RH

Culture System                  
Vero cell culture

Presentation           
Disrupted Toxoplasma "Ghosts" presented in 0.1M glycine buffer, pH 9.6

Inactivation             
Disruption
Confirmation by attempted growth under
original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Toxoplasma gondii IgM Antigen

Product Code                     
TOX-AG02

Agent            
Toxoplasma gondii Strain RH

Culture System                  
Vero cell culture

Presentation           
Disrupted Toxoplasma "Ghosts" treated with detergent and presented in cell culture medium containing detergent

Inactivation  
Disruption and detergent treatment
Confirmation by attempted growth under
original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Varicella Zoster Virus Antigen

Product Code                      
VZV-AG01

Agent            
Varicella Zoster Virus. Strain Ellen. ATCC VR-586

Culture System                  
Human Embryo Lung cell culture

Presentation           
Infected cells are harvested and virus extracted by alkaline treatment and sonnication. Clarified by centrifugation the preparation is further purified on a sucrose density gradient. The resulting antigen preparation consists of a high concentration of virus and viral components as well as some cellular material suspended in 0.1M glycine buffer, pH 9.6

Inactivation             
Beta-propiolactone treatment. Confirmation by attempted growth under original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided

Varicella Zoster Virus Glycoprotein Antigen

Product Code                     
VZV-AG02

Agent            
Varicella-Zoster Virus. Strain Ellen. ATCC VR-586

Culture System                  
Human Embryo Lung cell culture

Presentation           
Infected cells are harvested and antigen extracted by alkaline treatment, sonnication and detergent extraction. Glycoproteins are isolated by column chromatography using Lectin. The resulting antigen is suspended in 0.1M glycine buffer, pH 9.6

Inactivation             
Detergent treatment during processing. Confirmation by attempted growth under original culture conditions

Preservation           
None

Protein Concentration                  
mg/ml by Bradford (Biorad) assay using BSA as standard

Storage                    
-20 to -80°C. Repeated freezing and thawing should be avoided