Enzymes: Classifications

Recombinant Enzymes

Cat.No.	Description	Quantity
	DNA/RNA Enzymes
EN-001	DNA Gyrase, E.coli	1mg
EN-002	DNA Ligase, E.coli	10mg
EN-003	T4 DNA Ligase, phage T4	10mg
EN-802	Tth DNA Ligase	units
EN-004	T4 RNA Ligase, phage T4	10mg
EN-005	T4 DNA Polymerase, phage T4	10mg
EN-006	DNA Polymerase I holoenzyme, E.coli	1mg
EN-007	Exonuclease I, E.coli	10mg
EN-008	Exonuclease III, E.coli	10mg
EN-009	Exonuclease, T7 phage	10mg
EN-803	Klenow Fragment	units
EN-804	Real Script Reverse Transcriptase	units
EN-805	Real Script Plus Reverse Transcriptase	units
EN-806	RNAase-Inhibitor	units
EN-807	RNA Polymerase, T7 Phage	units
EN-808	RNA Polymerase, SP6	units
EN-010	Topoisomerase I, coli	10mg
EN-809	Tth inorganic pyrophosphatase	units
EN-810	Urail-DNA glycosylase	units
	Kinase
EN-101	Adenylate Kinase, E.coli	10mg
EN-102	Glucokinase, E.coli	10mg
EN-103	Galactokinase, E.coli	10mg
EN-104	Pyruvate Kinase, type F, E.coli	10mg
EN-105	Acetate Kinase, E.coli	10mg
EN-106	Glycerol Kinase, E.coli	10mg
EN-107	NAD Kinase, E.coli	10mg
EN-108	Nucleoside Diphosphate Kinase, E.coli	10mg
EN-811	T7 RNA polynucleotide  kinase	units
	Phospho-Enzymes
EN-109	Pyrophosphatase, Inorganic, E.coli	10mg
EN-110	Uridine Phosphorylase, E.coli	10mg
EN-111	Thymidine Phosphorlyase, E.coli	10mg
EN-112	Purine Nucleoside Phosphorylase, E.coli	10mg

Purity: All recombinant enzymes are tagged with polyhistidine and purified by metal chelate affinity chromatography. In addition, some of the enzymes are further purified chromatographically to achieve a high percentage purity. Enzymes are typically greater than 90% purity. The recombinant clones used to make the enzymes have been DNA sequence to confirm identity to the wild type enzyme (ensuring that the recombinant enzymes have no mutations). We do not assay enzymes for contaminating enzymes. Therefore, the customer should test the suitability of an enzyme for a particular application on a small scale prior to purchasing a bulk quantity of a lot. The enzymes produced by Bayou Biolabs are provided to customer "as is" without any warranty of merchantability or fitness for a particular purpose and without any other warranty, express or implied.

Supplied: Enzymes are supplied in glycerol buffer. Enzymes are shipped on dry ice at customer’s expense. Please see the table below for enzymes currently available. Bulk quantities: Bulk quantities are available at low cost. Standard bulk quantities are 100 mg, 500 mg, 1 gram, 5 grams, and 10 grams. We can also package any bulk quantity specified by customer. We have capability for scaling up to produce batches of several hundred grams. Please contact customer service for price and availability.

Custom production: We can custom produce milligram to multiple gram quantities of any protein or enzyme. In general, we use high fidelity PCR to amplify the gene of interest and clone the gene in our proprietary expression vector. We recommend using polyhistidine tags to facilitate enzyme purification, although we can also produce native and GST tagged proteins. Several recombinant clones are selected for DNA sequencing to obtain a clone with no mutations. Fermentation of the desired clone is performed at the desired scale (8 liters, 16 liters, 100 liters, 400 liters, 800 liters), and the protein is purified chromatographically according to the customer’s specifications, in order to obtain desired purity. Production can be customized to accommodate customer needs. Please contact customer service for a price quote.

Clinical Chemistry

Enzymes

Substrates