Product : Standard Preparation of Anti-HAV IgM

Cat.No. HRP-A601

Introduction:

Hepatitis A infection is caused by the hepatitis A virus. Hepatitis A virus is passed in the stool of infected persons. Unlike many other viruses, it can survive for a long time at room temperature in food and on surfaces and objects. The virus is not affected by cold or by freezing, but it is inactivated by heating foods to >185° F (85°C) for one minute. Adequate chlorination of water also kills the hepatitis A virus.The average time between exposure to the virus and the development of symptoms is about 28 days. Signs of illness usually begin abruptly. Infected persons with no symptoms can still spread the virus. This often happens with young children who unknowingly spread hepatitis A virus to older children and adults.

Hepatitis A can be diagnosed by a blood test called IgM anti-HAV.

Figure 1: Appearance of Hepatitis A marker after infection

Pentameric structure of IgM:

IgM in serum is composed of a pentamer of the basic 4 chain structure held together by inter H chain disulfide bonds. (Each basic unit has the same amino acid sequence as the others in the pentamer.) Thus, it has the ability to bind to 10 antigen at the same time. In addition, its heavy chain () is larger than the chain because it has four constant domains. Its molecular weight is about 65,000 daltons. Thus, the molecular weight of the monomeric form of IgM is about 180,000 daltons.

Polymerization is initiated by and the resulting pentamer is further stabilized by another molecule, the joining chain or J chain. J chain is a peptide of about 15,000 daltons which is rich in cysteines that are available to form disulfide bonds. IgM is so large it is normally confined to the intravascular space. One of the benefits of the inflammatory response is it lets IgM escape into the tissue spaces at the site where it may be needed to bind potential pathogens.

The structure of IgM is similar to that of IgG except the IgM heavy chain has an extra domain. A small, cysteine-rich protein called J chain initiates cross linking of C3 and C4 of five IgM monomers to make the circulating, pentameric form of IgM.

Product Features

1)      Source:                                                  

• Human Plasma/Serum

2)      Marker:                                                

• anti-HAV IgM positive

3)      Titer:                                                      

• >1:1.000
• >1:10.000
• >1:100,000

4)      Definition:                                              

• by a standardized anti-HAV IgM ELISA (anti-µ)

5)      Viral Markers:                                      

• anti-HIV I & II, HCV negative.

6)      Inactivation:                                           

• Heat inactivated at 60°C for 15 hours

7)      Volume:                                                 

• 100ml/500ml/1000ml/5000ml

8)      Storage:                                                 

• at -20°C

9)      Lot Number:                                          

• 070510

10)  Expiration Date:                                    

• 2 years after production.

REMARKS

Stability:               The stability of this material following freezing and thawing has not been evaluated. It is advisable to avoid repeated thawing/freezing of this material.

Safety:                  Please be aware that the material is heat-inactivated and might still contain infectious agents. In addition, because no test method can offer complete assurance that products derived from human source will not transmit infectious agents, it is recommended that this product be handled with the same precautions used for patient specimens.

Caution:               As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratory’s safety procedures. Such safety procedures probably will include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts. It is the responsibility of the user to ensure that he/she has the necessary technical skills to determine the appropriateness of this product for the proposed application. Results obtained from this product are likely to be dependent on conditions of use and the variability of materials beyond the control of DR.WANG.

Liability:              DR.WANG accepts no liability whatsoever for any loss or damage arising from the use of this product, whether loss of profits, or indirect or consequential loss. In particular, DR.WANG will not be liable for any damage resulting from handling or contact with the above product. All materials and mixtures may present unknown hazards and should be used with caution.