This kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative detection of antibodies (anti-HA) to hemagglutinin(HA) of Influenza A viruses, H5 strain (also known as highly pathogenic avian influenza) in human/avian serum or plasma samples. It is intended for clinical identification of infection with the H5 strain of type-A influenza viruses.
PRINCIPLE OF THE ASSAY
H5-HA(Ab) ELISA kit is based on solid phase, one step incubation competitive principle ELISA. H5 Influenza specific, anti hemagglutinin (anti-HA) antibodies, if present in the sample, compete with monoclonal Influenza H5 antibodies conjugated to horseradish peroxidase (anti-HA"mAb"HRP) for a fixed amount immunological Ag-Ab complex that has been pre-coated inside wells. This complex contains viral lysate of H5 HA, and monoclonal H5 specific anti-HA. When no H5 specific anti-HA presents in the sample, anti-HA"mAb"HRP will be bound with the H5 HA inside the wells and any unbound HRP-Conjugate is removed during washing. Chromogen A and B solutions are added into the wells and during incubation, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. No or low color developing suggests the presence of specific antibodies to hemagglutinin of influenza Type A, H5 strain.
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