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| Code No. | Description | Unit | Conc. |
| TAQ-001 | NormTaq DNA Polymerase | 100 U | 5U/µl |
| TAQ-002 | NormTaq DNA Polymerase | 500 U | 5U/µl |
| TAQ-003 | NormTaq DNA Polymerase | 2,500 | 5U/µl |
| TAQ-101 | NormTaq Opti DNA Polymerase | 100U | 5U/µl |
| TAQ-102 | NormTaq Opti DNA Polymerase | 500U | 5U/µl |
| TAQ-103 | NormTaq Opti DNA Polymerase | 2,500U | 5U/µl |
| TAQ-104 | NormTaq Opti DNA Polymerase | 5,000U | 5U/µl |
| TAQ-105 | NormTaq Opti DNA Polyemrase | 10,000U | 5U/µl |
| MMC-001 | NormTaq Color Master Mix | 100 Rx | |
| MMC-002 | NormTaq Color Master Mix | 1,000 Rx | |
| MMP-001 | PCR Master Mix | 100 Rx | 2x |
| MMP-002 | PCR Master Mix | 1,000 Rx | 2x |
Norm Taq DNA Polymerase provides improved amplification with convenience of reaction buffers containing magnesium. The 5 x reaction buffers supplied with this enzymes contain MgCl2 at a concentration of 7.5mM, giving a final concentration of 1.5mM in the 1 x reaction.
Norm Taq Opti DNA Polymerase allows you to optimize enzyme with magnesium concentration in your PCR. The supplied 5 x color reaction buffers do not contain magnesium. A separate tube of MgCl2 is supplied, giving you maximum control over your reaction conditions.
The 5X Color and 5X Colorless Reaction Buffers supplied with NormTaq enzymes allow you to go directly from thermal cycler to gel analysis. These buffers contain a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The colorn buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for post-amplification analysis by fluorescence or absorbance without prior purification of the DNA.
For ultimate convenience, choose PCR Master Mix or NormTaq Color Master Mix—premixed, ready-to-use 2X solutions containing NormTaq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. NormTaq Color Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. Reactions assembled with NormTaq Color Master Mix have sufficient density for direct loading onto agarose gels. Includes Nuclease-Free Water.
Storage Conditions Store enzymes at –20°C. PCR Master Mix can be stored at 4°C for 3 months; NormTaq Color Master Mix can be stored at 4°C for 6 weeks.
Patents/Disclaimers (a)Patents for the foundational PCR process, European Pat. Nos. 201,184 and 200,362, expired on March 28, 2006. In the U.S., the patents covering the foundational PCR process expired on March 29, 2005.
