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Hepatitis E Virus (HEV) GENE DETECTION KIT
Product Code: GT-NTE101
Intended Use
The kit is developed for the detection of viral RNA of the hepatitis E virus by reverse transcriptase/polymerase chain reaction (RT-PCR*).
Assay principle
Hepatitis E is an infectious viral disease with clinical and epidemiological features of acute hepatitis. Only seldom adult patients progress to fulminant hepatitis, but among pregnant patients the case fatality rate can be as high as 20%-30%. Hepatitis E is a waterborne disease transmitted primarily by contaminated water. There is also a possibility of zoonotic spread of the virus, since several non-human primates, pigs, cows, sheep, goats and rodents are susceptible to the infection. Hepatitis E virus (HEV) is a principal cause of acute hepatitis in adults throughout much of Asia, Middle East and Northern Africa and transmitted from person-to-person through the fecal-oral route. At least four major genotypes of HEV have been identified, only one serotype is recognized. HEV is endemic in developing countries where sanitary conditions are not well maintained.
After RNA extraction and reverse transcription viral RNA can be detected in stool and in serum of the infected patient by PCR*. For the detection of very low virus titers as it is regularly the case in serum samples it is required to perform a nested PCR*.
The HEV genotypes differ from each other; therefore, the kit contains two sets of reaction mixes. It is recommended to assay the samples with both sets of reaction mixes.
Target RNA and Amplified Fragments
- Viral RNA of HEV
- Amplicon RT-PCR* A 348 bp
- Amplicon RT-PCR* B 500 bp
Contents:
The kit contains the lyophilised reaction mixes for running 20 specific RT reactions with primer A, 30 PCR* and 30 nested PCR* reactions with RxMix A, as well as 20 specific RT reactions with primer B, 30 PCR and 30 nested PCR* reactions with RxMix B.
Required materials not provided:
- Taq-Polymerase and Reverse Transcriptase
- Agarose
- TAE- or TBE electrophoresis buffer (can be included if required)
- Sample buffer (can be included if required)
- DNA-Standard (can be included if required)
- Ethidiumbromide or other dyes for nucleic acid staining
- Mineral oil for thermocyclers without heating lid
Related products
- HEV Antigen ELISA
- HEV Gene Sequencing Kit
- HEV Genotyping Kit
- HEV Human IgG ELISA
- HEV Human IgA ELISA
- HEV Human IgM ELISA
- HEV Pig IgG ELISA
*PCR: Hoffmann-LaRoche Inc. owns the U.S.-Patent for performing PCR. The performance of PCR may be carried out under license of Hoffmann-LaRoche Inc.. The provided information with this test-kit does not include an authorization to perform PCR nor a license for the performance. The user of this kit performs the PCR on his own responsibility to obtain the necessary license.
