Kits

Code	Description	Storage	Size
MB01	Random Primer DNA Labeling Mix; A one step premixed solution for the labeling of DNA with radiolabeled dCTP, using random nonaprimer oligonucleotides. Labeling obtained within 10 minutes.	-20°C	25 X
MB02	DNA Isolation Kit; An easy-to-use kit for the extraction of DNA from agarose gels. The pure DNA fragments can be used in ligations, PCR, cloning and probe construction. The entire purification procedure takes only 30 minutes. The kit can be used for the isolation of plasmid DNA from mini-preps and for the concentration of DNA without ethanol precipitation.	+4°C	150-300 X
MB03	DNA Genomic DNA Isolation Kit;  This is a non-organic and ready-to-use reagent for the isolation of genomic DNA from samples of human, animal, plant, yeast, bacterial and viral origin. The kit is based on disruption of cells in a guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from a cell lysate with ethanol. Following an ethanol wash. DNA is solubilized in water or 8 mM NaOH. There is no phenol in the kit. The protocol is fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. The procedure can be completed in 10-30 minutes with DNA recovery of 70-100%. The isolated DNA can be used, without additional purification, for Southern analysis, dot blot hybridization, molecular cloning, RFLP, PCR and other molecular biology and biotechnology applications.	AMB	50ml
MB04	RNA Total RNA Isolation Kit; This is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. It is based on disruption of cells in guanidine thiocyanate/detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and allows simultaneous processing of a large number of samples. The resulting RNA is suitable for the isolation of Poly A+ RNA or for Northern Blotting, Dot Blotting, in vitro Translation, Molecular Cloning, RT-PCR and RNase Protection Assays, or other analytical procedures. DNA and proteins can also be recovered from the interphase and the organic phase of the same sample.	+4°C	100ml
MB04	RNA II Total Isolation Kit without Chloroform (with BCP); This is a complete kit with ready-to-use reagents for the isolation of total RNA from samples of human, animal, plant, yeast, bacterial and viral origin. The kit is based on disruption of cells in guanidine thiocyanate/detergent solution, followed by organic extraction and alcohol precipitation of the RNA, and which allows simultaneous processing of a large number of samples. 1-Bromo-3-chloropropane (BCP) replaces chloroform, a highly volatile and toxic reagent used in molecular biology. Substituting BCP for chloroform in the EZ-RNA II kit reduces toxic material handling without any reverse effects on the quality of isolated RNA, DNA or proteins. The resulting RNA is suitable for the isolation of Poly A+ RNA or for Northern Blotting, Dot Blotting, in vitro Translation, Molecular Cloning, RT-PCR and RNase Protection Assays, or other analytical procedures. DNA and proteins can also be recovered from the interphase and the organic phase of the same sample.	+4°C	100ml
MB06	First Strand cDNA Isolation Kit (For RT-PCR); Premixed solutions for the synthesis of single-stranded cDNA from RNA for use as a PCR template Using the First Strand cDNA Synthesis Kit, RNA is reverse-transcribed into single-stranded cDNA. The reverse transcriptase (RT) enzyme synthesizes the new cDNA strand at a site determined by the type of primer used: oligo(dT) primer, random primer or a sequence-specific primer. The first strand cDNA can then be used as a template for PCR.  The reaction mix solution supplied contains buffer, MMLV reverse transcriptase (point mutant lacking RNase H activity), and RNase inhibitor sufficient for 50 reactions.	-20°C	50 X
MB07	ECL Kit; Chemiluminescence Detection Kit for HRP ECL is a complete kit with ready-to-use reagents for chemiluminescence detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Southern or Northern) conjugated with HRP (Horse Radish Peroxidase) directly or indirectly. Using this method, it is possible to detect membrane immobilized specific antigens, or sequences of nucleic acids, labeled directly with HRP or indirectly with HRP-labeled antibodies/streptavidin. Advantages: ·          High sensitivity non-radioactive detection system ·          Stable hard copy results on film ·          Only small amounts of antibody required ·          High resolution	+4°C	120ml

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