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MORE SCIENCE, NO FICTION
Imagine what you could accomplish with an extra pair of hands. And what about another pair of eyes? While the concept sounds like science fiction, the reality is that you can stretch your productivity with the newest life science instruments and reagents. Ingenious technologies literally allow you to do and see more. You can maximize every assay and every hour you spend at the bench.
Time is not the only advantage of products. The strategy allows researchers to acquire a bird’s eye view of gene or protein expression and the personalized signatures of disease—such as the “immense natural heterogeneity in tumor phenotypes, disease outcomes, and response to therapies,” ...
Fluorescent and non-radioactive tracer microspheres for determination of regional blood flow distribution and particle deposition: a safe, environment-friendly, accurate and cost-effective technique!
Fluorescent Micropheres
These polystyrene microspheres are monodisperse and uniform, ranging from 0.1µm to 4 µm and are also available as plain or functionalized microspheres. These fluorescent microspheres contain a variety of dyes, spanning the visible spectrum from UV (Excitation wavelength = 375nm/ Emission wavelength = 435nm) to red (Excitation wavelength. = 420nm / Emission wavelength = 660nm)
Our Fluorescent Microspheres are supplied at 1% concentration in water (100mg/10ml).
Underlined wavelengths represent predominant excitation and emission wavelengths.
X is excitable at the 442nm He-Cd laser line.
XC and Y are well suited to the 488nm and 515nm bands of the Argon laser.
Fluorescent Standard Microspheres for Flow Cytometer
New type of Fluorescent and Monodisperse Microspheres have been developed and are designed for the routine calibration and control for flow cytometry.The fluorescent particles have a long term photo-stability and are supplied as ready-to-use reagents.
Molecular Imaging
In addition, fluorescent microspheres are a sensitive non-radioactive method of measuring regional blood flow by dye extraction. After recovery of the microspheres from the harvested tissue samples, the dye is extracted and quantified by fluorescence spectrophotometry. Fluorescent microspheres have been well documented in the literature.
- Comparison between radioactive and non radioactive microspheres
Radioactive microspheres have been used for many years as the standard for measuring regional blood flow distribution in animal research models. Radioactive microspheres have many negative health, safety and environmental issues associated with their use – furthermore, the disposal of research material must wait for 23 half-lives of the most longest lived isotope used. Non-radioactive microspheres are safe, environment-friendly and research material does not require specific disposal methods.
Our microspheres have been thoroughly tested by independent investigators. The testing has included the stability of dye attachment, completeness of dye recovery, and comparisons with radioactive microsphere results. The studies demonstrated that regional blood flow can be accurately measured over a wide range of flow in different species using this cost effective, non-radioactive technique.
- Main functionalities of non-radioactive microspheres
We offer a variety of non-radioactive tracer microspheres for determination of regional blood flow distribution and particle deposition. Uniform-sized tracer microspheres are made of stable polystyrene latex. These microspheres are marked with one of several unique families of markers and used to measure regional blood flow at different time points during the course of an experiment. Typically blood flow measurements are made using 15um diameter microspheres. Occasionally 10um microspheres are also used for blood flow applications. Various other sizes of tracer microspheres are available for other applications in a range of sizes from 3 to 100um.
- Typical research applications for non-radioactive spheres
Microspheres find applications in a very large spectrum of research fields. They can be used for any study where volume blood flow is of interest. Following table offers an non exhaustive overview of typical applications for various domaines.
| Brain | Differences in blood flow between the right and left brain, differences in blood flow to different areas of the brain |
| Kidney | Differences between the right and left kidney, differences in blood flow within each kidney, studies of acute renal failure |
| Heart | Regional blood flow differences for the myocardium or across the ventricular wall, myocardial ischemia studies, mapping of regions of the myocardium receiving reduced blood flow, studies of the relationship between blood flow and myocardial function, studies of blood flow changes stimulated by angiogenic drugs |
| Liver | Studies of liver blood flow including changes due to shunting, damage or injury |
| Skeletal muscle | Studies of muscle blood flow in response to exercise |
| Bones | Studies of blood flow to normal bones, as well as broken bones that are healing |
| Skin | Studies of blood flow to normal, damaged or burned skin |
| Lung/Airway | Studies of blood flow to normal or diseased lungs, studies of airflow into the lungs and airways |
| Fetal | Studies in blood flow to and within the fetus |
| Trauma | Blood flow to any of the above tissues due to induced trauma or injury |
| Medical Devices | Testing devices that augment or control blood flow |
| Pharmacology | Studies of the effects of drugs to regional blood flow in any of the tissues listed above |
Kit for Aligning and Focusing Flow Cytometers
• Size of particles : 2 – 2.5 µm
• Argon-ion laser excitation : 488 nm
• Emission range : 500 – 660 nm
• CV fluorescence : < 2 %
An Intensity Calibration Kit (different beads suspensions of various % relative fluorescent intensities) recommended for calibrating.
• Size of particles : 2 – 2.5 µm
• Argon-ion laser excitation : 488 nm
• Emission range : 500 – 660 nm
• CV fluorescence : < 4 %
Kit for Aligning Flow Cytometers at Different Wavelengths.
| Excitation (nm) | Emission (nm) |
| 360 | 450 |
| 488, 514 | 585 - 610 |
| 360, 488, 514 | 560 - 670 |
| Dye | Excitation Wavelength (nm) | Emission (nm) Color |
| U | Scintillator or eventually photoescitable at 375 | White |
| X | 440, 455, 468, 480, 510 | Yellow |
| XC | 470, 490, 525, 560 | Pink |
| Y | 515, 555, 568, 610 | Red |
| Z | 420, 515, 550, 656, 720 | Red |
Plain Polystyrene Fluorescent Microspheres
| Code | Product# | Diameter (µm) | Polymer | |
| FM-002 | F-U030 | 0,276-0,325 | Polystyrene | |
| FM-003 | F-U050 | 0,476-0,575 | Polystyrene | |
| FM-004 | F-U100 | 0,900-1,100 | Polystyrene | |
| FM-005 | F-U200 | 1,800-2,200 | Polystyrene | |
| FM-006 | F-U300 | 2,700-3,300 | Polystyrene | |
 | FM-007 | F-X010 | 0,076-0,125 | Polystyrene |
| FM-008 | F-X050 | 0,476-0,575 | Polystyrene | |
| FM-001 | F-X050 | 0,076-0,125 | Polystyrene | |
| FM-009 | F-X100 | 0,900-1,100 | Polystyrene | |
| FM-010 | F-X200 | 1,800-2,200 | Polystyrene | |
| FM-011 | F-X300 | 2,700-3,300 | Polystyrene | |
| FM-012 | F-XC010 | 0,076-0,125 | Polystyrene | |
| FM-013 | F-XC030 | 0,276-0,325 | Polystyrene | |
| FM-014 | F-XC050 | 0,476-0,575 | Polystyrene | |
| FM-015 | F-XC100 | 0,900-1,100 | Polystyrene | |
| FM-016 | F-XC200 | 1,800-2,200 | Polystyrene | |
| FM-017 | F-XC300 | 2,700-3,300 | Polystyrene | |
| FM-018 | F-Y010 | 0,076-0,125 | Polystyrene | |
| FM-019 | F-Y030 | 0,276-0,325 | Polystyrene | |
| FM-020 | F-Y200 | 1,800-2,200 | Polystyrene | |
| FM-021 | F-Y300 | 2,700-3,300 | Polystyrene | |
| FM-022 | F-Z010 | 0,076-0,125 | Polystyrene | |
| FM-023 | F-Z030 | 0,276-0,325 | Polystyrene | |
| FM-024 | F-Z050 | 0,476-0,575 | Polystyrene | |
| FM-025 | F-Z100 | 0,900-1,100 | Polystyrene | |
| FM-026 | F-Z 200 | 1,800-2,200 | Polystyrene | |
| FM-027 | F-Z 300 | 2,700-3,300 | Polystyrene |
COOH-Modified Fluorescent Microspheres
| Code | Product# | Diameter (µm) | Polymer | |
| FM-028 | F1-U010 | 0,130-0,190 | Polystyrene | |
| FM-029 | F1-U030 | 0,270-0,330 | Polystyrene | |
| FM-030 | F1-U050 | 0,450-0,530 | Polystyrene | |
| FM-031 | F1-U080 | 0,760-0,940 | Polystyrene | |
| FM-032 | F1-U100 | 0,950-1,100 | Polystyrene | |
| FM-033 | F1-X010 | 0,130-0,190 | Polystyrene |
| FM-034 | F1-X030 | 0,270-0,330 | Polystyrene | |
| FM-035 | F1-X050 | 0,450-0,530 | Polystyrene | |
| FM-036 | F1-X080 | 0,760-0,940 | Polystyrene | |
| FM-037 | F1-X100 | 0,950-1,100 | Polystyrene | |
| FM-038 | F1-XC010 | 0,130-0,190 | Polystyrene | |
| FM-039 | F1-XC030 | 0,270-0,330 | Polystyrene | |
| FM-040 | F1-XC050 | 0,450-0,530 | Polystyrene | |
| FM-041 | F1-XC080 | 0,760-0,940 | Polystyrene | |
| FM-042 | F1-XC100 | 0,950-1,100 | Polystyrene | |
| FM-043 | F1-Y010 | 0,130-0,190 | Polystyrene | |
| FM-044 | F1-Y030 | 0,270-0,330 | Polystyrene | |
| FM-045 | F1-Y050 | 0,450-0,530 | Polystyrene | |
| FM-046 | F1-Y080 | 0,760-0,940 | Polystyrene | |
| FM-047 | F1-Y100 | 0,950-1,100 | Polystyrene | |
| FM-048 | F1-Z010 | 0,130-0,190 | Polystyrene | |
| FM-049 | F1-Z030 | 0,270-0,330 | Polystyrene | |
| FM-050 | F1-Z050 | 0,450-0,530 | Polystyrene | |
| FM-051 | F1-Z080 | 0,760-0,940 | Polystyrene | |
| FM-052 | F1-Z100 | 0,950-1,100 | Polystyrene |
Kit for Aligning Flow Cytometers at Different Wavelengths
| Excitation (nm) | Emission (nm) |
| 360 | 450 |
| 488, 514 | 585 - 610 |
| 360, 488, 514 | 560 - 670 |
Please contact us to find the microsphere solution matching your research needs
Developing New Diagnostic Tests
Fluorescent beads coated with different HLA antigens have been incubated with patient sera to detect anti-HLA antibodies. The left panel shows the analysis of beads incubated with a negative serum, while the right panel shows the same beads incubated with serum that contains multiple anti-HLA antibodies.Werlen, G., Hausmann, B.T., Naeher, D. and Palmer, E. Life and death decisions in the thymus: Timing is everthing. Science 199: 1859-63 (2003).
Molecular Evolution of Biological Complexity: Fluorescent Proteins
Phylogenetic tree of the coral fluorescent proteins drawn on a Petri dish with bacterial cultures expressing resurrected ancestral proteins and present-day proteins (from Ugalde, Chang and Matz, Science 2004). Note that the complex red color evolved from the simple green ancestor in a gradual fashion. Ugalde, J. A., Chang, B. S. W. and Matz, M. V. Evolution of coral pigments recreated. Science 2004, 305: 1433.
Fluorescent Substances/Proteins as Biotechnology Tools
The first application of fluorescent proteins from reef organisms for dual-color in vivo labeling: tracing the progeny of left and right dorsal blastomeres in a developing tadpole (from Matz et al, Nature Biotechnol 1999). Left: white light; right: fluorescent optics.Matz, M., Fradkov, A., Labas, Y., Savitsky, A., Zaraisky, A., Markelov, M. and Lukyanov, S. Fluorescent proteins from non-bioluminescent Anthozoa species. Nature Biotechnol. 1999, 17: 969-973.
Molecular Imaging: Analysis of Tumor Vessel Supply in Lewis Lung Carcinoma in Mice by Fluorescent Microsphere Distribution and Imaging with Micro- and Flat-Panel Computed Tomography American Journal of Pathology. 2005;167:937-946.
CUSTOM DYED MICROSPHERES
If you cannot find a product to meet your specific requirements in our standard range of products, then you can probably have them made to order. Virtually any of our polymer microspheres can be supplied dyed with your choice from a wide range of plain and fluorescent dyes.
For further information on this service, please contact: dr.wang@t-online.de
