DNA/EcoR I markers are designed for sizing double-stranded DNA on 1% to 2% agarose gel electrophoresis and can be visualized after ethidium bromide staining. They are prepared from completely digested DNACI857 S7with EcoRI, purified and then dialyzed. These fragments are applicable for radiolabeling.

Storage buffer: 10 mM Tris-HCl pH 7.8, 1 mM EDTA, 10 mM NaCl

Concentration: 625 ug/ml

Recommended storage condition: -20°C

Fragments: 21226’, 7421, 5804, 5643, 4878 and 3530’ bp. An additional band form 24756 bp, may from fragments of 21226 and 3530 bp, indicated by ’. Separation of these fragments is achieved by heating at 65°C

Additional buffer: 5x loading dye